human sdf 1 duoset elisa  (R&D Systems)

Journal: Scientific reports

Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells.

doi: 10.1038/s41598-025-02977-x

Figure Lengend Snippet: Fig. 2. CXCL12 induces GSC chemotaxis in a biomimetic device. (A) Design of the negative mold for the biomimetic device and development of the PDMS device. (B) Time-lapse images between T = 0 and T = 42 h for dextran release from our hyaluronic acid/collagen II-based (HA/Col) hydrogel in the device using Zen 2.5D visualization software (Zeiss). (C) Time-lapse images of GSC migration in the device in the presence or absence of GliaTrap + CXCL12. Images were acquired each day for 4 consecutive days. GSC spheroids were embedded in the middle chamber of the device in Collagen I. (D) Coordinates of each migrating GSC in control or GliaTrap devices at day 4 were plotted using R. (E) Quantification of the ratio of migrating GSCs between the left and right side of the middle line of each GSC spheroid. GliaTrap induces significant increase in the number of GSCs migrating towards the GliaTrap containing chamber of the device (*p < 0.05, Student’s t-test). (F) Coordinate single cell migration data were used to calculate the polar coordinates and degrees from the origin and visualized as a rose plot using the R package ggplot2.

Article Snippet: CXCL12 released amount was determined using a human CXCL12 ELISA kit (R&D systems) after breaking liposomes using 1% of Triton X-100.

Techniques: Chemotaxis Assay, Software, Migration, Control

Journal: Scientific reports

Article Title: GliaTrap is a biodegradable, non-swelling and non-inflammatory hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells.

doi: 10.1038/s41598-025-02977-x

Figure Lengend Snippet: Fig. 4. GliaTrap does not induce inflammatory infiltration in vivo. Mouse brain coronal Sect. (4 μm thickness) at the level of injection site across three experimental groups: subhippocampal needle stick only (A–E), injection of hydrogel vehicle only (F–J), and injection of hydrogel containing CXCL12 (GliaTrap) (K–O). Representative images are shown, chosen from among n = 3 mice in each experimental group. From left to right, slices were prepared with H&E staining; F4/80 mAb 1:250 to identify macrophages; CD4 mAb 1:100 to identify CD4 + T-Cells; CD8a mAb 1:400 to identify CD8 + T-Cells; and Granzyme B mAb 1:100 to identify activated T cells and natural killer cells. All antibodies and concentrations were the same as for spleen positive control staining from the injection-only mice, as shown in (Supplementary Fig. 3). Antibody positivity was observed in F4/80 in all three groups adjacent to the injection site (purple arrows), but not in slices prepared using the other three antibodies. Images were captured using an Axio Observer Z1/7 microscope, Axiocam 705 1X Camera Adapter, and EC Plan-Neuofluar 10X / 0.30 M27 Objective.

Article Snippet: CXCL12 released amount was determined using a human CXCL12 ELISA kit (R&D systems) after breaking liposomes using 1% of Triton X-100.

Techniques: In Vivo, Injection, Staining, Positive Control, Microscopy